One England star appeared to avoid shaking hands with Thomas Partey moments before the World Cup clash with Ghana.
Ahead of kick-off in Boston, the two teams lined up for traditional pre-match handshakes following the respective national anthems.
BBC cameras panned away from the handshakes just as the Ghana squad began walking down the England line.
Former Arsenal midfielder Partey had already made his way down the line by the time cameras returned to focus on the players.
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But footage shared on social media from within the stadium appeared to show Tottenham defender Spence refusing to shake Partey’s hand.
The midfielder was heavily booed by sections of the England support during the earlty stages of the match.
Last July, London’s Metropolitan Police charged Partey with five counts of rape and one count of sexual assault. In February, the 32-year-old was charged with two additional counts of rape.
Partey is awaiting trial and has pleaded not guilty to all charges.
England captain Harry Kane was among those pictured shaking the Villarreal player’s hand.
Last week, it was reported the Football Association would not issue any guidance to the squad over shaking hands with the Ghana player.
Partey was released by Arsenal at the end of his contract last summer, joining Spanish side Villarreal.
He missed Ghana’s opening match against Panama last week after being denied a Canadian visa.
‘It’s part of football,” Partey said. ‘Things happen outside football that you cannot control. For me now, I feel OK and ready to play.’